Adipose-derived stem cells combined with platelet-rich plasma enhance wound healing in a rat model of full-thickness skin defects

Abstract Background Wound healing is impaired in patients with diabetes due to the multifactorial etiology of disease, which limits therapeutic efficacy various approaches. This study hypothesizes that combination adipose-derived stem cells (ADSCs) and platelet-rich plasma (PRP) might achieve optimally efficient diabetic wound healing. Methods ADSCs were isolated from adipose tissues Sprague-Dawley (SD) rats. PRP was prepared by using a two-step centrifugation technique. A model established on backs SD rats evaluate effect incorporated into PRP. Hematoxylin eosin staining, immunofluorescence, immunohistochemistry performed observe changes neovascularization. ELISA Western blot utilized detect angiogenesis-related protein expression levels. The proliferation endothelial assessed MTS assay. Results induced higher closure rate than ADSCs, PRP, negative control. levels VEGF, p-STAT3, SDF-1 ADSC+PRP group those other groups. Moreover, strongly stimulated treatment ADSC-conditioned medium (ADSC-CM) Conclusions enhanced its promoting involved